Table 2.

Effect of Ala mutations on the kinetic parameters of the Hill equation for ATP saturation curves, as determined with and without 1.5 mM FBP.^a

		ATP
	FBP (mM)	Vmax (U/mg)	S0.5 (mM)	n
WT	1.5	88.0 ± 0.6	0.17 ± 0.01	2.0 ± 0.1
	0	21.8 ± 0.8	2.5 ± 0.1	2.4 ± 0.2
P103A	1.5	23.7 ± 0.8	0.81 ± 0.06	1.5 ± 0.1
	0	18.3 ± 0.2	0.89 ± 0.02	1.9 ± 0.1
Q105A	1.5	52.0 ± 1.9	0.74 ± 0.06	1.4 ± 0.1
	0	13.0 ± 0.3	1.91 ± 0.05	2.3 ± 0.1
Q106A	1.5	32.2 ± 4.5	4.4 ± 1.1	1.2 ± 0.1
	0	11.1 ± 0.6	2.5 ± 0.2	1.7 ± 1.6
R107A	1.5	27.2 ± 0.6	1.63 ± 0.05	2.3 ± 0.1
	0	16.8 ± 0.5	2.5 ± 0.1	2.1 ± 0.1
M108A	1.5	42.6 ± 2.0	0.31 ± 0.03	2.2 ± 0.5
	0	21.5 ± 0.3	2.54 ± 0.06	1.8 ± 0.1
K109A	1.5	55.7 ± 0.5	0.38 ± 0.01	2.2 ± 0.1
	0	16.0 ± 0.3	2.11 ± 0.06	1.5 ± 0.1
E111A	1.5	66.7 ± 1.9	0.29 ± 0.02	1.3 ± 0.1
	0	15.5 ± 1.5	3.3 ± 0.5	1.7 ± 0.2
N112A	1.5	41.9 ± 2.0	0.43 ± 0.05	1.7 ± 0.3
	0	13.3 ± 1.7	1.7 ± 0.3	1.7 ± 0.4
W113A	1.5	15.0 ± 2.3	3.1 ± 1.2	1.1 ± 0.1
	0	11.3 ± 1.0	3.5 ± 0.7	1.2 ± 0.1
Y114A	1.5	14.1 ± 1.5	2.3 ± 0.3	1.9 ± 0.3
	0	10.1 ± 0.3	2.5 ± 0.1	2.0 ± 0.1
R115A	1.5	13.2 ± 0.7	1.3 ± 0.1	1.7 ± 0.2
	0	10.1 ± 1.3	3.0 ± 0.5	2.1 ± 0.5

^aEnzyme assays were performed as described in Materials and Methods.