Table 2.
Residue POOL Rankings, POOL log ZP Score, and Loss of Catalytic Efficiency for PGI Variants
| Variant | Shell | POOL rank | POOL log ZP score | (kcat/KM) wild type/(kcat/KM)mutant | Reference |
|---|---|---|---|---|---|
| H389L | First | 1 | 2.0 | >10,000 | 24 |
| H396L | Third | 3 | 1.3 | 24 | 24 |
| (R273A) | First | 6 | 0.79 | (170,000) | 39 |
| Y341F | Third | 11 | 0.66 | 1.5 | 24 |
| (E358Q) | First | 13 | 0.59 | (2,100) | 40 |
| E495Q | Second | 14 | 0.58 | 250 | 24 |
| (E217Q) | First | 20 | 0.49 | (130,000) | 40 |
| H100L | Third | 21 | 0.48 | 630 | 24 |
| Y274F | Second | 23 | 0.34 | 2.2 | 24 |
| N386A | Second | 25 | 0.22 | 2.8 | 24 |
| K362A | Second | 26 | 0.27 | >10,000 | 24 |
| Q388A | Second | 29 | 0.18 | 19 | 24 |
| (K519A) | First | 39 | 0.06 | (390) | 39 |
| (Q512A) | First | 44 | 0.02 | (120) | 40 |
| (K211A) | First | 45 | 0.01 | (19) | 39 |
| D511N | Second | 57 | −0.03 | 230 | 24 |
| N154Q | Second | 135 | −0.08 | 3.8 | 24 |
| S185A | Second | 140 | −0.08 | 3.5 | 24 |
| (K440A) | Third | 162 | −0.08 | (1.0) | 39 |
Parentheses indicate kinetics data reported for the homologous residue in the B. stearothermophilus enzyme (with hPGI numbering). All other kinetics data are for human PGI.