Table 3.
Residue POOL Rankings, POOL log ZP Score, and Change in Catalytic Efficiency for P. putida KSI Variants
| Variant | Shell | POOL rank | POOL log ZP score | (kcat/KM)wild type/(kcat/KM)mutant | Reference |
|---|---|---|---|---|---|
| Y32F | Second | 1 | 1.9 | 1.3 | 54 |
| Y32S | “ | “ | “ | 3.6 | 55 |
| Y57F | First | 2 | 1.4 | 2.9 | 54 |
| Y57S | “ | “ | “ | 66 | 55 |
| Y16F | First | 3 | 1.4 | 550 | 54 |
| Y16S | “ | “ | “ | 18 | 55 |
| D40N | First | 4 | 1.1 | 334,000 | 56 |
| W120F | First | 5 | 0.20 | 13 | 57 |
| F56L | First | 6 | 0.13 | 3.6 | 57 |
| D103N | First | 9 | 0.074 | 27 | 51 |
| E39Q | Second | 12 | 0.015 | 2.4 | 24 |
| F86L | First | 14 | −0.006 | 9.2 | 57 |
| M105A | Third | 16 | −0.026 | 7.6 | 24 |
| M84A | Second | 23 | −0.05 | 0.98 | 24 |
| M31A | Second | 34 | −0.05 | 2.1 | 24 |
| C97S | Second | 39 | −0.05 | 1.3 | 58 |
| C81S | Second | 48 | −0.05 | 0.98 | 58 |
| C69S | Second | 58 | −0.05 | 0.92 | 58 |