Table 4.

Residue POOL Rankings, POOL log ZP Score, and Change in Catalytic Efficiency for E. coli Alkaline Phosphatase Variants

Variant	Shell	Pool rank	POOL log ZP score	(kcat/KM) wild type/(kcat/KM)mutant	Reference
D51N	First	1	2.8	12	66
D369N	First	2	2.6	95	19
D327N	First	4	2.5	100	67
D327A	First	4	“	>1,000,000	67
H412N	First	6	2.0	130	68
H331E	First	7	1.2	972	65
D101S	First	8	1.0	0.2	63
H372A	Second	9	1.0	0.27	18
H372D	Second	9	“	3.3	This work
H372L	Second	9	“	2.5	This work
D153G	First	10	0.72	0.2	63
D153N	First	10	“	1.1	64
R166A	First	11	0.60	313	69
K328A	First	13	0.47	3.8	70
H86L	Second	14	0.45	3.2	This work
E57Q	Third	17	0.17	1.8	This work
T155M	First	18	0.16	678	19
S102A	First	19	0.16	150,000	71
M53A	Second	21	0.13	1.5	This work
M53T	Second	21	“	2.4	This work
D330N	Second	22	0.06	0.2	21
D330N	Second	22	“	2.1	This work
Q435E	Second	44	−0.03	1.7	This work
T100V	Second	51	−0.03	0.3	20
V99A	Second	100	−0.06	0.2	20
E150Q	Second	106	−0.06	3.7	This work
S105A	Second	136	−0.06	2.1	This work

Residue numbering follows the PDB file 1ALK.