Table 2.
Calorimetric Data for NICD–CSL Binding Between Mouse and Drosophila Components
| Cell | Syringe | K (M−1) | Kd (µM) | ΔG° (kcal/mol) | ΔH° (kcal/mol) | −TΔS° (kcal/mol) | |
|---|---|---|---|---|---|---|---|
| Su(H) + mNICD | mRAMANK | Su(H) | 4.9 ± 0.8 × 106 | 0.206 | −9.1 ± 0.09 | −17.7 ± 0.8 | 8.6 ± 0.8 |
| mRAM | Su(H) | 2.5 ± 1.1 × 106 | 0.437 | −8.7 ± 0.2 | −14.6 ± 0.7 | 5.9 ± 0.9 | |
| mANK | Su(H) | NBD | NBD | NBD | NBD | NBD | |
| Su(H) | mANK | NBD | NBD | NBD | NBD | NBD | |
| dCTD | mANK | NBD | NBD | NBD | NBD | NBD | |
| RBP-J + dNICD | dRAMANK | RBP-J | 2.8 ± 1.6 × 107 | 0.050 | −10.0 ± 0.4 | −16.6 ± 0.5 | 6.5 ± 0.9 |
| dRAM | RBP-J | 2.9 ± 0.5 × 107 | 0.040 | −10.1 ± 0.1 | −14.9 ± 0.2 | 4.7 ± 0.3 | |
| dANK | RBP-J | NBD | NBD | NBD | NBD | NBD | |
| RBPJ | dANK | NBD | NBD | NBD | NBD | NBD | |
| mANK | mCTD | NBD | NBD | NBD | NBD | NBD | |
| mCTD | dANK | NBD | NBD | NBD | NBD | NBD |
All experiments were performed at 25°C. Values are the mean of at least three independent experiments and errors represent the standard deviation of multiple experiments. NBD, no binding detected.