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. 2015 Apr 2;24(5):861–873. doi: 10.1002/pro.2660

Table 1.

Sequence assignment by tandem mass spectrometry of selected peaks generated from limited trypsin digestion of MBP-core RAG1a

m/z Peptide Sequencebc Peptide
1730 1) 762-SNPYHESVEELRDR-775 e
2) 90-LYPFTWDAVRYNGK-103 (MBP)d
2094 1) 950-DGSIGAWASEGNESGNKLFR-969 f
2) 622-FSFTVMRITIEHGSQNVK-639
2387e 1) 559-YDSALVSALMDMEEDILEGMR-579 c1
2) 807-IFQLEIGEVYKHPNASKEER-826 c2
2464 1) 509-VLLPGYHPFEWQPPLKNVSSR-529 b
2) 757-YEVWRSNPYHESVEELRDR-775
a

The table lists the m/z value of each analyzed peak (first column), the sequences of two different theoretical trypsin-generated MBP-core RAG1 peptides (labeled 1 and 2) with m/z values within ± 1 D of the value in first column (second column), and the peptide labels b, c1, c2, e, and f consistent with the labels in Figure 1B (third column).

b

The peptides in boldface indicate the sequences detected by tandem mass spectrometry. The sequences in light gray font were not detected, resulting in unambiguous assignments for three of the peaks (peptides b, e, and f).

c

The residue numbers are shown for the first and last peptide residue. The numbers for the core RAG1 peptides correspond to that for the full length murine RAG1 protein.

d

Corresponds to a peptide in the MBP portion of the fusion protein.

e

The peak with an m/z value of 2387 consisted of a mixture of both of the peptides listed (labeled as c1 and c2).