Table 2.

Effect of heme oxygenase (HMOX) induction on bile salt-dependent and -independent bile flow

Group of animals	Biliary bile acids output (μmol/g/min.)	Bile flow (μl/g/min.)	Biliary glutathione output (nmol/g/min.)
Vehicle (CON)	210.02 ± 43.59	2.21 ± 0.25	7.43 ± 2.34
EE-treated (EE)	81.64 ± 8.94*	0.54 ± 0.10*	0.32 ± 0.04*
Vehicle + heme (HC)	207.24 ± 12.03	2.13 ± 0.47	9.38 ± 0.17
EE + heme (HE)	135.52 ± 45.07*†	0.81 ± 0.25*†	0.52 ± 0.05*†

Bile volume, bile acids and glutathione concentrations were measured in the bile collected for 30 min. from control (CON), ethinylestradiol (EE), heme (HC) or heme + ethinylestradiol (HE)-treated animals and recalculated to grams of liver tissue.

^*P ≤ 0.05 versus CON

^†P ≤ 0.05 versus EE.