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. 2015 Mar 6;19(5):986–995. doi: 10.1111/jcmm.12487

Figure 4.

Figure 4

Chemerin activated the AKT-FoxO3α signalling pathway in a CMKLR1-dependent manner. (A) Forty-eight hours after transfection with CMKLR1 siRNA, the cells were pre-treated with or without chemerin (0.5 μg/ml) for 24 hrs, stimulated with insulin (100 nmol/l) for 15 min., solubilized in SDS sample buffer and analysed by western blotting with antibodies against p-AKT and p-FoxO3α. (Β) After the transfection of CMKLR1 siRNA, the cells were solubilized in SDS sample buffer and analysed by western blotting with antibodies against FoxO1 and FoxO3α. Left: representative western blot image; right: quantification of the protein expression. The results are presented relative to the values in the control cells. The data are presented as the means ± SEM from four independent experiments. *P < 0.05 versus the control group; #P < 0.05 versus the chemerin group.