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. 2015 Feb 20;19(5):1055–1064. doi: 10.1111/jcmm.12498

Figure 1.

Figure 1

Cytotoxic activity of aurantiamide acetate on in vitro cultured human malignant glioma U87 and U251. (A) U251 and U87 cells were seeded into 96-well plates at a density of 1000 cells/well. Twenty-four hours after initial seeding, cells were treated with different concentrations of aurantiamide acetate (0, 10, 25, 50 or 100 μM). Following different time period (0, 24, 48 or 72 hrs) of drug incubation, the cell viability was determined by MTT. (B) U87 cells were seeded into 96-well plates at a density of 500 cells/well (low density) or 1500 cells/well (high density). Twenty-four hours after initial seeding, cells were incubated with aurantiamide acetate (0, 10, 25 or 50 μM). Following different time period (0, 48 or 72 hrs) of drug incubation, the cell viability was determined by MTT. (C) Representative cellular morphology after 48-hrs incubation of 25 μM aurantiamide acetate (AA). Dead cells were indicated by arrows. (D) The number of processes per cell was quantified from six micrographs. At least 30 cells per micrograph were analysed. (E) Cells treated with 25 μM AA or 2.5 μg/ml cisplatin (Cis) for 48 hrs were stained with Hoechst 33342. Apoptotic cells were indicated by arrows; scale bars, 50 μm. (F) The proportion of cells exhibiting condensed apoptotic nuclei was calculated from 20 micrographs. *P < 0.05; **P < 0.01 compared with control.