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. 2015 Feb 20;19(5):1055–1064. doi: 10.1111/jcmm.12498

Figure 2.

Figure 2

Influence of aurantiamide acetate on intracellular organelles of U87 cells. Cells were transfected with EGFP-Mito (A), EYFP-Golgi (E) or EGFP-Lgp120 (F) to visualize the alternations of mitochondria, Golgi apparatus or lysosomes. Some of the cells were stained with MitoTracker Red (B–D) to determine mitochondrial membrane potential or co-stained with Phalloidin and PI (G) to label F-actin and nucleus respectively. Normal healthy U87 cells (Ctrl) or cells following 48-hrs of 25 μM aurantiamide acetate incubation (AA) were used for analysis of the intracellular organelle alternation. Boxed area in (A) was enlarged in the same figure. BF, bright field. Scale bars in (A), (B) and (G), 20 μm; scale bars in (E) and (F), 10 μm. Quantified data were shown in (C) and (D). Data were calculated from 12 micrographs. *P < 0.05 compared with control.