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. 2015 Feb 20;19(5):1055–1064. doi: 10.1111/jcmm.12498

Figure 5.

Figure 5

Influence of autophagy stimulator or inhibitors on aurantiamide acetate-induced cell death. U87 cells were incubated with 25 μM aurantiamide acetate (AA), 10 nM rapamycin (Rapa), 10 mM 3-methyladenine (3-MA), 40 nM Bafilomycin A1 (BafA1), 10 μM chloroquine (CQ), 25 μM AA plus 10 nM Rapa, 25 μM AA plus 10 mM 3-MA, 25 μM AA plus 40 nM BafA1, or 25 μM AA plus 10 μM CQ. Cells without drug treatment were used as control (Ctrl). (A) Representative images were presented; scale bar, 50 μm. (B) The number of processes per cell was quantified from six micrographs. At least 30 cells per micrograph were analysed. *P < 0.05; **P < 0.01 compared with control; ##P < 0.01 compared with AA.