Figure 1.

Dengue virus (DV) infection activated Gal-9 in human DCs. Human DCs (1 × 10⁶ cells/ml) were infected by mock or DV NGC strain at an MOI of 5. Quantitative RT-PCR was used to determine expression of MRNAs of the Gal-9, Gal-1 and Gal-3 genes (A), and Gal-9 protein levels were determined by Western blotting (B). Cells were infected by mock or DV (NGC strain) at various MOIs for 48 hrs, and Gal-9 expression was determined by Western blotting (C). Similarly, protein levels of Gal-9 in human DCs infected by different DV2 strain 16681 (D) or PL046 (E) at an MOI of 5 for various time-points were determined. The data shown are from three independent experiments examining different donor DCs.