Skip to main content
. 2015 Mar 6;19(5):1065–1076. doi: 10.1111/jcmm.12500

Figure 3.

Figure 3

Defective migration of DV-infected DCs deficient in Gal-9. Human DCs were transfected with different siRNAs (si-Ctl, si-G9-1 or si-G9-2) for 24 hrs and then infected with mock or DV for 48 hrs. Expression of Gal-9 protein and Gal-9 mRNA was determined by Western blotting (A) and quantitative RT/PCR (B), respectively. In addition, the supernatants were collected for the determination of Gal-9 concentrations (C). Cells transfected with control siRNA (si-Ctl) or Gal-9 siRNA (si-G9-1) for 24 hrs were infected by mock or DV for additional 48 hrs. Cells were collected for measurement of chemotactic activity by transwell assays using CCL19 (D) or CCL21 (E) as a chemoattractant. In (F), the cells were treated with Gal-9 recombinant protein (rGal-9, 10 μg/ml) or PBS for 48 hrs and then collected for determination of chemotactic activity by transwell assays. DV-infected cells treated with control siRNA transfection (D and E) or rGal-9-treated cells (F) were defined as 100%. The data show results pooled from at least three independent experiments examining different donor DCs. The analysis was performed by anova. *P < 0.05, **P < 0.01, ***P < 0.001. Ctl, control.