Skip to main content
. 2015 Mar 6;19(5):1065–1076. doi: 10.1111/jcmm.12500

Figure 4.

Figure 4

DV infection-induced production of IL-12 was Gal-9- and p38-dependent. Human DCs were transfected with control siRNA (si-Ctl) or Gal-9 siRNA (si-G9-1) for 24 hrs and were then infected by mock or DV for an additional 48 hrs. The supernatants were collected for determination of levels of IL-12p40 (A), MIP1-β (B), MCP-2 (C), IFN-α (D), TNF-α (E), IL-1β (F), IL-6 (G), TGF-β1 (H) and IL-12p70 (I) by ELISA. In (J–L), before infection by DV, DCs were pretreated with various doses of the p38 inhibitor SB203580 (J), the ERK inhibitor PD98059 (K), or the JNK inhibitor SP600125 (L) for 2 hrs. The data show results pooled from at least three independent experiments. The analysis was performed by anova. *P < 0.05, **P < 0.01, ***P < 0.001. Ctl, control.