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. 2015 Mar 6;19(5):1065–1076. doi: 10.1111/jcmm.12500

Figure 6.

Figure 6

Defective CCL19-mediated activation of ERK in DV-infected DCs with knockdown of Gal-9. Human DCs transfected with control siRNA (si-Ctl) or Gal-9 siRNA (si-G9-1) for 24 hrs were infected by mock or DV for an additional 48 hrs. Cells were collected for measurement of CCR7 expression by flow cytometry (A). Before collection, uninfected DCs and DCs infected with mock or DV were treated with CCL19 200 ng/ml for 2 min. (for determination of p-ERK expression), CCL19 500 ng/ml for 5 min. (for determination of p-p38 expression) or CCL19 500 ng/ml for 30 min. (for determination of p-JNK expression), and the cell lysates were collected individually to determine expression of total and activated MAPKs by Western blotting (B). The results pooled from at least three independent experiments using different donor cells are presented next to the representative figures. The analysis was performed by anova. *P < 0.05, **P < 0.01, ***P < 0.001. Ctl, control; ns, non-significant; MFI, mean fluorescence intensity.