Figure 3.
QL suppressed the transcription of IL-6 through regulating calcineurin/NFAT3 signalling pathway. CFs were stimulated by AngII (100 nmol/l) for 24 hrs with or without the pre-treatment of QL (0.5 mg/ml), (A) the cytoplasm level of calcineurin and (B) nuclear level of NFAT3 were detected by Western blot, respectively, in separated protein extracts. (C) CFs were pre-treated with or without FK506 (10−5 mol/l) for 1 hr before AngII stimulation, the effects of QL and FK506 on IL-6 mRNA was detected in different groups. (D) Actin stress fibres were detected by a-SMA staining in CFs from different treated groups (control, AngII, AngII with FK506, AngII with QL, AngII with FK506 and QL). (E) Quantification of mean fluorescence intensity of stress fibres in CFs with a-SMA staining. *indicates P< 0.05 versus AngII-induced group.