Skip to main content
. 2015 Feb 26;19(5):1133–1150. doi: 10.1111/jcmm.12518

Figure 2.

Figure 2

Immunoblot analysis of autophagy and stress-response proteins in BMSCs challenged with Staphylococcus epidermidis. The protein extracts were obtained from the BMSC cultures 24 hrs after a challenge with S. epidermidis, except the cells pre-treated with 10 μM PDTC and 200 nM Wortmannin (see Materials and methods) were collected at 5 hrs post-challenge. (A) Immunoblot bands of stress-responding proteins. (B) Densitometrical analysis of the protein bands shown in A; the displayed data are ratio of the protein band densities to the densities of the respective actin bands. (C) Immunoblot bands of autolysosomal proteins. (D) Densitometrical analysis of Atg12-Atg5 and LC3(II) bands shown in C; the displayed data are ratio of the protein band densities to the densities of the respective actin bands. (E) Immunoblot bands of mitochondrial and mitophagy-related proteins. (F) Densitometrical analysis of PINK1 and PARK2 bands shown in E; the displayed data are ratio of the protein band densities to the densities of the respective actin bands.