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. Author manuscript; available in PMC: 2016 May 5.

Published in final edited form as: Insect Biochem Mol Biol. 2014 Nov 5;55:39–50. doi: 10.1016/j.ibmb.2014.10.007

(A) The expression of cpp-miR-71 as analysed by stem-loop quantitative RT-PCR after microinjection the negative control mimic and cpp-miR-71 mimic and DEPC. In the cpp-miR-71 mimic microinjection group, the expression level was 22.63 fold higher than with the DEPC microinjection. (B) qRT-PCR analysis of CYP325BG3 after microinjection. Compared with DEPC-water group, the target gene CYP325BG3 was knocked down almost 57% with the cpp-miR-71 microinjection. (C) Mortality of microinjected mosquitoes observed after a two hour exposure to CDC bottles treated with deltamethrin (4 mg/ml). The results were presented as mean± standard error (SE) of three independent experiments (*p<0.05, **p<0.01). There was no statistically significant difference between the DEPC and negative control (NC) mimic microinjection group.

(A) The expression of cpp-miR-71 as analysed by stem-loop quantitative RT-PCR after microinjection the negative control mimic and cpp-miR-71 mimic and DEPC. In the cpp-miR-71 mimic microinjection group, the expression level was 22.63 fold higher than with the DEPC microinjection. (B) qRT-PCR analysis of CYP325BG3 after microinjection. Compared with DEPC-water group, the target gene CYP325BG3 was knocked down almost 57% with the cpp-miR-71 microinjection. (C) Mortality of microinjected mosquitoes observed after a two hour exposure to CDC bottles treated with deltamethrin (4 mg/ml). The results were presented as mean± standard error (SE) of three independent experiments (*p<0.05, **p<0.01). There was no statistically significant difference between the DEPC and negative control (NC) mimic microinjection group.