Figure 3.

Decreased frequency of CD69⁺ CD4⁺ T cells in draining LNs of Cbl-b^−/− mice after CFA/MOG_35–55 immunization. WT and Cbl-b^−/− mice were immunized as in Figure 2 and the popliteal LNs were harvested 4–5 days later. (A) Percentage of CD4⁺ T cells (left) and CD8⁺ T cells (right) that are CD69⁺ in the popliteal LNs of WT and Cbl-b^−/− mice. (B) Percentage of CD4⁺ T cells (left) and CD8⁺ T cells (right) that are CD69⁺ in the mLNs of WT and Cbl-b^−/− mice. *p<0.05, ****p<0.0001 by Student’s t-test. The data shown are from three independent experiments.