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. Author manuscript; available in PMC: 2015 May 5.
Published in final edited form as: Mol Nutr Food Res. 2015 Mar 18;59(5):853–864. doi: 10.1002/mnfr.201400819

Figure 6. Resveratrol induces apoptosis in activated BMDM and lowers TNFα release of BMDM induced by LPS.

Figure 6

Using an in vitro assay, BMDMs were pre-incubated with LPS (100 ng/ml) for 2 h, then various concentrations of resveratrol (10, 20, 50, 100 µmol) were added. After an additional 24 h, the medium and cells were collected for follow-up assay. A. Apoptosis was evaluated by flow cytometry. B. The percentage of apoptotic cells was determined by Annexin V/PI staining. The cells that were positive for Annexin V, PI, or both were considered apoptotic. C. The concentration of TNFα in the medium was measured by ELISA. Data were presented as mean ± SEM. (n=6, in triplicate; #p<0.05 vs. Control; *p<0.05 or **p<0.01 vs. LPS only).