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. Author manuscript; available in PMC: 2015 May 5.
Published in final edited form as: Mol Nutr Food Res. 2015 Mar 18;59(5):853–864. doi: 10.1002/mnfr.201400819

Figure 7. Resveratrol modulates LPS-stimulated macrophages by TLR4-NF-κB signaling.

Figure 7

A. Using an in vivo assay, mice were treated as above, and the macrophages isolated from the kidneys of mice from each group. The protein was prepared for western blot to evaluate the expression levels of TLR4 and p-IκBα in cell lysates using relevant antibodies. β-actin was used as a standard control. NF-κB-p65 was used to evaluate the activity of NF-κB in nuclear extracts and Lamin-B was used as a standard control. The groups were designated as follows, LPS: LPS injected group; RES: Resveratrol only group; RES + LPS: LPS + Resveratrol pre-treatment group; LPS + RES: LPS + Resveratrol post-treatment group. B. In vitro assay, BMDM were treated as described in Figure 6, the expression level of TLR4 and p-IκBα in cell lysates using relevant antibodies, β-actin was used as a standard control. The fold change was compared with the LPS group in vivo or the LPS only group in vitro (as 1.0).