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Potential of mean force for acetamide as a function of pair distance at different insertion depth into the lipid bilayer from simulations with A) explicit solvent and lipids B) HDGB implicit membrane C) GBSW implicit membrane and D) IMM1 implicit membrane models; For each insertion depth, the bound state was used as the reference with an energy of zero. The white bars indicate a lack of sampling overlap along the z direction.
