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. 2015 May 5;10(5):e0125323. doi: 10.1371/journal.pone.0125323

Fig 4. Local injection of 2HP-β-CD enhances blood flow recovery (A), and stimulates angiogenesis and arteriogenesis (B).

Fig 4

(A) Ischemic/non-ischemic limb blood flow ratio assessed by LDBF imager in mice that received different concentrations of 2HP-β-CD for 28 days. Data represent the mean ± S.E.M. (n = 8 mice per group). ** p < 0.01 and *** p < 0.001 versus the PBS-treated mice. (B) 2HP-β-CD-stimulated angiogenesis and arteriogenesis in C57BL/6 mice. EC marker CD31 (red) and SMC marker αSMA (green) were detected by double immunofluorescence in the ischemic hindlimb of PBS-treated (upper panel) and 2HP-β-CD-treated (lower panel) mice. Representative immunofluorescence images of each marker and merged images are shown. (C) CD31 positive microvessels, αSMA positive vessels and αSMA positive vascular area were quantified using Image J software. Data represent the mean ± S.E.M. (n = 4 mice per group). * p < 0.05 versus the PBS-treated control mice. Scale bar = 50 μm.