Fig. 1.

Fasting induced a rapid loss of fat but not muscle, as revealed by X-ray CT analysis. (A) Representative scanned whole body images of ad libitum-fed (AL), 1-day fasted, and 2-day fasted mice are shown. Representative lumber vertebra 1–2 (L1–L2) slice images are also shown, in which green and red regions represent fat and muscle (non-fat) depots, respectively. (B and C) Fat (B) and muscle (C) contents in the lower half of the body were estimated. Data are shown as the mean ± S.D. (n = 8), and significant changes were found in the fat tissues but not muscles; ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 (Mann–Whitney U-test).