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Western blot analysis of various mouse tissue homogenates for LC3b and p62 expression. Anti-LC3B antibody detected both LC3-I (∼18 kDa) and LC3-II (∼16 kDa) bands while anti-p62 and anti-GAPDH antibodies detected ∼62 kDa and ∼38 kDa bands, respectively. AL, ad libitum-fed; F1, 1-day fasting; F2, 2-day fasting; S.I., small intestine; S.M., skeletal (rectus femoris) muscle.
