Fig. 7.

Fasting-induced tissue-specific expression changes in genes related to the Nrf2-mediated antioxidant system. The expression of HO-1 (A) and Gsta1 (B) in various tissues was analyzed by qPCR and normalized to Hprt expression. The expression ratios in ad libitum-fed (AL) mice were set at 1. Data are shown as the mean ± S.D. (n = 4), and the differences were significant at ^∗P < 0.05 (Mann–Whitney U-test). F1, 1-day fasting; F2, 2-day fasting; S. intestine, small intestine; S. muscle, skeletal (rectus femoris) muscle. Highly atrophied (>31% weight decrease), moderately atrophied (18–19% weight decrease), and non-atrophied tissues (no significant change) are labeled in red, green, and black, respectively.