Table 2.
Changes in wet tissue weights by 1- or 2-day of fasting.
| Weight at day 0 (mg tissue) | % decrease at day 1 | % decrease at day 2 | |
|---|---|---|---|
| Body weight (n = 13 each) | 23.9 ± 0.8 (g) | –16.7 ± 4.6∗∗∗ | –23.4 ± 2.9∗∗∗,### |
| Tissues | |||
| Brain (n = 13 each) | 427.9 ± 28.8 | –2.8 ± 5.3 | –2.7 ± 9.4 |
| Eye (n = 13 each) | 43.4 ± 4.5 | –6.3 ± 12.0 | (+)1.2 ± 7.6 |
| Thymus (n = 13 each) | 50.2 ± 12.2 | –33.5 ± 17.2∗∗ | –54.7 ± 11.7∗∗∗,## |
| Lung (n = 13 each) | 146.9 ± 32.4 | –1.6 ± 16.9 | –8.8 ± 19.9 |
| Heart (n = 13 each) | 120.0 ± 21.1 | –6.3 ± 15.4 | –8.2 ± 18.1 |
| Liver (n = 9 each) | 1197.7 ± 92.7 | –26.9 ± 6.4∗∗∗ | –32.2 ± 4.0∗∗∗ |
| Stomach (n = 13 each) | 174.0 ± 33.8 | –13.6 ± 16.8 | –18.0 ± 17.1∗ |
| Kidney (n = 13 each) | 322.3 ± 40.4 | –13.2 ± 13.5∗ | –18.6 ± 7.0∗∗∗ |
| Spleen (n = 13 each) | 74.4 ± 6.2 | –26.0 ± 10.6∗∗∗ | –41.2 ± 13.2∗∗∗,## |
| Pancreas (n = 6 each) | 122.2 ± 16.2 | –25.6 ± 8.8∗∗ | –37.4 ± 7.4∗∗,# |
| Small intestine (n = 6 each) | 780.6 ± 52.9 | –23.2 ± 6.0∗∗ | –31.6 ± 3.2∗∗,# |
| Colon (n = 6 each) | 119.4 ± 20.8 | (+)7.2 ± 5.6 | –18.0 ± 11.9∗,## |
| Skeletal muscle (n = 6 each) | 274.7 ± 26.8 | –5.6 ± 3.8 | –4.6 ± 12.0 |
| Testis (n = 13 each) | 208.6 ± 45.2 | –7.5 ± 18.6 | –9.2 ± 15.1 |
C57BL/6J male mice were fed ad libitum, or fasted for 1 or 2 day(s) between 2 pm and 2 pm with free access to water. Tissues were isolated after anesthetization and weighed. Blood in the heart and the contents of the stomach, small intestine, and colon, were washed out with ice-cold phosphate-buffered saline before weighing. Isolated rectus femoris muscles were weighed as skeletal muscle. Data are means ± S.D. (n: sample numbers); significant changes (decreases in weights) in ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus day 0; #p < 0.05 and ##p < 0.01 versus day 1 (Mann–Whitney U-test).