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. 2015 May 6;35(18):7082–7094. doi: 10.1523/JNEUROSCI.3778-14.2015

Figure 1.

Figure 1.

Paranodal localization of BK channels in the rat cerebellum. A, Non-uniform compact localization of BK channels in axons in the granule layer of the cerebellum. Rat cerebellar sections were immunolabeled with the L6/60 mouse mAb against BK (Slo1.1, red) and an antibody against an axonal marker, unphosphorylated neurofilament (Axon, green). Insets show magnified views of the immunolabeling. Scale bar, 50 μm. B, Two mAbs against BK recognize two distinct epitopes. Immunoblot of recombinant full-length BK channels and fragments representing residues 859–1169 and residues 664–865, probed with L6/60 and L6/48 mAbs. C, The compact localization of BK channels in axons verified by immunolabeling with two distinct mAbs (L6/60, red; L6/48, green). Scale bar, 5 μm. D, Double immunolabeling with L6/60 (red) and an anti-Caspr antibody (green) showing their colocalization in the paranode. Scale bar, 5 μm. E, Immunolabeling for Nav1.6 (green) is present in the gap between the paired paranodal BK channel immunolabeling (red). Scale bar, 5 μm.