Figure 5.

(A) Horizontal brain section, where the left CP and SN only were targeted with FUS, immonostained for p-Ret, and counterstained with hematoxylin, (B) is image (A) after the extraction of the DAB color only corresponding to p-Ret. (C, D, G, and H) are higher magnification at the targeted and contralateral CP and SN. (E, F) and (I, J) are the corresponding images after the extraction of the DAB color only corresponding to p-Ret. At the targeted with FUS side shown in (E) and (I), there is an increased p-Ret compared with the contralateral side shown in (F) and (J), respectively. Both sides were exposed to NTN through the systemic circulation for 1 hour after the intravenous administration. Immunostaining is detected in the neuronal bodies as well as in the dendrites and axon terminals, providing information on the location of the Ret receptor in the dopaminergic (DA) neurons. CP, caudoputamen; DAB, diaminobenzidine; FUS, focused ultrasound; NTN, neurturin; SN, substantia nigra.