Fig. 1.

Relative expression of thromboxane synthase (Tbxas) and thromboxane A2 receptor (Tbxa2r) in mice and a macrophage cell line. A–F: expression of Tbxas and Tbxa2r mRNA expression in epididymal white adipose tissue (eWAT) of lean wild-type (WT; n = 10) and leptin-deficient obese (ob/ob; n = 10) mice (A and B); eWAT (C and D), subcutaneous (inguinal) white adipose tissue (iWAT; E and F), liver, and skeletal muscle (Tbxa2r only; G) of mice fed a low-fat diet (LFD; n = 7) or high-fat diet (HFD; n = 7); and isolated primary adipocytes or cells of the stromal vascular fraction (SVF; n = 3) (H and I). J and K: expression of Tbxas and Tbxa2r mRNA expression in eWAT and iWAT of chow-fed male mice under fasted, refed, or ad libitum conditions (n = 10/group). L and M: expression of Tbxas (L) and Tbxa2r (M) mRNA in cultured RAW264.7 macrophage cells treated with recombinant IL-1β (2 ng/ml), IL-6 (2 ng/ml), TNFα (2 ng/ml), IFNγ (50 ng/ml), or resistin (100 ng/ml) for 6 h (n = 6). Expression data were normalized to 18S rRNA in each sample. All data are expressed as means ± SE. *P < 0.05 compared with ad libitum group; **P < 0.01; ***P < 0.001.