FIG 1.

Bipartite topology of TP_0326 (BamA) in T. pallidum. (A) Following encapsulation in gel microdroplets, intact (I) T. pallidum or organisms permeabilized (P) with 0.05% Triton X-100 were probed with rat antisera directed against the β-barrel or POTRA regions of TP_0326 or FlaA. Antibody binding was detected with Alexa Fluor 488-conjugated goat anti-rat antibody (green). Scale bars = 2.5 μm. (B) Mean percentages of labeling ± SEMs from three independent gel microdroplet experiments. Statistical significance (I versus P) was assigned according to the following scheme: ***, P < 0.0001.