FIG 3.

Growth profile of E. coli wild-type and mutant strains expressing B. subtilis YrvO and/or MnmA. (A and B) E. coli MG1655 (circles) and an E. coli ΔiscS strain expressing either B. subtilis yrvO (triangles), yrvO(C325A) (inverted triangles), yrvO and mnmA (diamonds), or yrvO(C325A) and mnmA (right triangles) or the empty expression vector (squares) were cultured in minimal medium, and optical density measurements were taken as indicated. (C and D) E. coli BW25113 (circles) and an E. coli mnmA-deficient strain expressing B. subtilis mnmA (triangles) or yrvO and mnmA (diamonds) or the empty expression vector (squares) were cultured in minimal medium, and optical density measurements were taken as indicated. For growth analysis of all strains tested, cultures were grown in M9 minimal medium in the absence (A and C) or presence (B and D) of isoleucine, leucine, methionine, cysteine, thiamine, and nicotinic acid at 37°C and 300 rpm. When not visible, error bars are smaller than the symbols.