FIG 2.

Colony size phenotypes of the B. anthracis Sterne glutamate racemase mutants. (A) B. anthracis Sterne and the ΔracE1, ΔracE2::aad9, and complemented ΔracE2::aad9/pRacE2 variants were spread on BHI agar and incubated at 23, 30, 37, and 42°C or on LB agar and incubated at 30°C. The diminished colony size of the racE2 mutant at 30 and 37°C was complemented by plasmid-borne racE2 (pRacE2). (B) The diminished colony size of the racE2 mutant was also complemented by plasmid-borne racE1 (pRacE1).