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Cell division defect of germinated racE2 mutants. Spores (1 × 106/ml) derived from B. anthracis and the ΔracE2::aad9 mutant were inoculated into BHI broth. Aliquots were withdrawn at timed intervals, fixed with formalin, and stained with BODIPY-vancomycin to identify cell wall septa (arrows) and with FM4-64 to reveal membranes and then viewed by fluorescence microscopy. Scale bar, 5 μm.
