FIG 10.

Growth (A) and uptake of [¹⁴C]linoleic acid (B) following exposure of S. aureus USA300 and USA300 farE::ΦNE to an increase in concentration of linoleic acid. In panel A, quadruplicate cultures of USA300, USA300 farE::ΦNE(pLI50), or USA300 farE::ΦNE(pLIfarE) were grown in TSB–20 μM linoleic acid to an OD₆₀₀ of approximately 0.2 to 0.3. The cultures were then supplemented with an additional 50 μM dose of linoleic acid, and growth (OD₆₀₀) was measured after 30 min and then at hourly intervals. When this experiment was conducted for the purpose of quantifying uptake of [¹⁴C]linoleic acid, the cultures were supplemented with 0.20 μCi/ml of [¹⁴C]linoleic acid ([¹⁴C]-LA) at the 30-min time point, and aliquots of culture were processed for quantification of [¹⁴C]linoleic acid uptake at intervals of 1, 2, 5, and 10 min. Each data point represents the mean and standard deviation of values of quadruplicate samples.