TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| S. aureus | ||
| USA300 LAC | Community-associated MRSA, wild-type strain cured of resistance plasmids | 24 |
| RN4220 | rK− mK+, capable of accepting foreign DNA | 32 |
| NE1393 | Transposon insertion in SAUSA300_2490, Ermr | 34 |
| NE2336 | Transposon insertion in SAUSA300_2489, Ermr | 34 |
| USA300 farR::ΦNE | USA300 LAC recipient of transposon from NE1393 | This study |
| USA300 farR::ΦNE(pLIfarR) | farR::ΦNE complemented with native farR, cloned in pLI50; Ermr Cmr | This study |
| USA300 farR::ΦNE(pCNfarR) | farR::ΦNE complemented with pCNfarR for cadmium-inducible expression, Ermr Cmr | This study |
| USA300 farE::ΦNE | USA300 LAC recipient of transposon from NE2336 | This study |
| USA300 farE::ΦNE(pLIfarE) | farE::ΦNE complemented with native farE, cloned in pLI50 | This study |
| USA300 farE::ΦNE(pLI50) | USA300 farE::ΦNE with empty pLI50 vector, Cmr | This study |
| USA300 Δtet38 | USA300 LAC with internal deletion of tet38 (SAUSA300_0139) | This study |
| USA300 Δtet38-farE::ΦNE | USA300 Δtet38 recipient of farE::ΦNE transposon insertion, Ermr | This study |
| E. coli DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Invitrogen |
| Plasmids | ||
| pLI50 | E. coli-S. aureus shuttle vector | 35 |
| pLIfarR | pLI50 with native farR gene | This study |
| pLIfarE | pLI50 with native farE gene | This study |
| pGYlux | E. coli-S. aureus shuttle vector harboring promoterless luxABCDE operon | 37 |
| pCN51 | E. coli-S. aureus shuttle vector with Pcad promoter for cadmium-inducible gene expression | 36 |
| pCN51c | pCN51 with ermC cassette replaced by cat194 cassette from pRN7146 | This study |
| pCN51farR | pCN51c with promoterless farR for cadmium-inducible expression of farR | This study |
| pKOR-1 | E. coli-S. aureus shuttle vector; contains Pxyl-tetO, expresses antisense secY RNA | 38 |
| pKORΔtet38 | pKOR-1 containing upstream and downstream flanking sequences for deletion of tet38 | This study |