Transcriptional activity of candidate DNA MTases in OG1RF. cDNA was used as the template for RT-qPCR with primers targeting predicted DNA MTases and the control gene, clpX. Expression values were quantified relative to clpX. Negative control reactions were performed with RNA template and clpX primers. Blue bar, OD600 = 0.05; orange bar, OD600 = 0.35; purple bar, OD600 = 1.0; yellow bar, OD600 = 2 to 2.2. Bars represent averages of two independent growth curve experiments, with standard deviations shown.