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. 2015 May 5;197(11):1939–1951. doi: 10.1128/JB.00130-15

FIG 4.

FIG 4

OG1RF_11622 (M.EfaRFI) is responsible for 5′-GCWGC-3′ methylation. gDNAs from OG1RF (lanes 1 and 2 for undigested control and digested sample, respectively), OG1RF ΔEfaRFI (lanes 3 and 4), OG1RF ΔEfaRFI pAT28 (lanes 5 and 6), and OG1RF ΔEfaRFI pM.EfaRFI (lanes 7 and 8) were digested with ApeKI. gDNA (600 ng) was digested in a 50-μl reaction volume, and 10-μl samples were analyzed by agarose gel electrophoresis. DNA size standards are shown on the left. TseI digestions yielded identical results (data not shown).