FIG 1.

Ccr4-Not and DST1 mutations display synthetically enhanced phenotypes. (A) Single and double Ccr4-Not and dst1Δ mutants were spotted onto SC medium or SC medium containing 5 μg/ml MPA. The plates were incubated at 30°C for either 2 or 4 days, as indicated. (B) Schematic of the constructs used in the GLAM assay. (C) GLAM assay. Strains were grown to log phase in YP medium supplemented with galactose and raffinose. Northern blotting using probes directed to GAL1 (short) and YLR454W (long) was performed, and the ratio of the transcripts was calculated for each strain and compared to that for the wild type (WT), which was set equal to 1.0. Data are presented as the averages and standard deviations from 4 assays.