FIG 4.

Differential PM segregation of H-Ras oncogenic forms. (A) Presence of oncogenic and wt H-Ras forms in epithelial cells. (Top) The presence of wt H-Ras and H-RasL61 is distinguished by differential mobility in high-concentration SDS-polyacrylamide gels; (bottom) the presence of H-Ras–GTP was analyzed by Raf-RBD pulldown. H-Ras–GTP/total H-Ras levels relative to those in MCA3D cells are presented beneath the gel. (B) H-Ras distribution in epithelial cells displaying different H-Ras genotypes.