FIG 6.

PM sublocalization of palmitoylation-defective Ras proteins. (Left) Representative FRET micrographs prebleaching (pre) and postbleaching (post) of HeLa cells transfected with wild-type (H wt), monopalmitoylated C184S mutant (H 184), and unpalmitoylated C181/184S (H SS) H-Ras–Cerulean constructs, in addition to the DRM-specific FRET probe LCK-Venus. (Insets) Bleached area. Bar, 10 μm. (Right) Quantitation of FRET efficiencies. Results show the mean ± SEM from at least five experiments. ***, P < 0.001 with 95% confidence intervals.