FIG 9.

The H-Ras shift between PM microdomains does not require a functional GC. (A) Effects of BFA on the GC and on H-Ras sublocalization. The immunofluorescence of endogenous H-Ras subcellular localization in control MEFs and MEFs treated with BFA (100 μM, 1 h) is shown. DAPI staining shows nuclei, and GC is revealed using antigiantin antibodies. Confocal sections at the level of the nuclei are shown. Bar, 10 μm. (B) BFA treatment does not impede the H-Ras shift induced by APT-1 overexpression. Figure 7C shows the results for the control not treated with BFA. (C) FRET analyses of the effects of BFA on APT-1-induced H-Ras PM sublocalization in MEFs. Results show the mean ± SEM from at least five experiments. *, P < 0.05; NS, not significant. (D) Distribution of N-Ras in serum-starved cells. (E) The distribution of N-Ras in MEFs is unaffected by the overexpression of APT-1 and DHHC9/GCP16.