FIG 4.

Western immunoblot analysis for phosphorylation of the SGK1 substrates, NDRG-1 and FoxO3a, in cells overexpressing SGK1. SH-SY5Y cells were infected with AdV-SGK1 and incubated for 48 h for protein expression as described in Materials and Methods. α-Tubulin is shown as a loading control. Data presented are representative of Western blots from three biological replicates. MW, molecular weight (in thousands).