FIG 7.

SRC expression reconstitutes metastasis in miR-1 expressing DU145/RasB1 cells. (A) Bone metastasis scores per mouse in tumor-bearing mice fed normal or doxycycline diets. Mice were inoculated with DU145/RasB1/miR-1 cells expressing Tet-regulated SRC. ***, P < 0.001. (B) Representative bioluminescence images (top), histology sections (middle), and radiographs of bone metastases (bottom) in the mice used for panel A compared with DU145/RasB1. Bone metastases are indicated by arrowshead. Black arrowheads, adjacent osteolysis; blue arrowheads, adjacent new bone formation. (C) Survival rates of the tumor-bearing mice used for panel A. **, P < 0.01. (D) BLI analysis of tumor burden in bones of the tumor-bearing mice used for panel A compared with DU145/RasB1.