TABLE 2.
Antibodies used for Western blot analysisa
| Primary-antibody target | Source | Dilution |
|---|---|---|
| p-ERK1/2 | Cell Signaling (4376) | 1/2,000 |
| ERK2 | Cell Signaling (9102) | 1/1,000 |
| SRC | Cell Signaling (2109) | 1/2,000 |
| ZEB1 | Novus (NBP1-05987) | 1/500 |
| ETS1 | Epitomics (T3123-1) | 1/2,000 |
| GAPDH | Novus (NB300-221) | 1/4,000 |
| p-Erk2 | Cell Signaling (4370) | 1/1,000 |
| Erk2 | Santa Cruz (1647) | 1/1,000 |
| α-Tubulin | Sigma (T8203) | 1:2000 |
a
Horseradish peroxidase-linked anti-mouse IgG (NXA931; 1:2,000) or anti-rabbit (NA934; 1:2,000) IgG (GE Health Care) was used for protein detection.