FIG 3.

Sam68 associates in vitro with RNA elements in Rps6kb1 intron 6. (A) Sequence spanning mouse Rps6kb1 exon 6, intron 6, which contains the three alternative exons (6a, 6b, and 6c) and exon 7, taken from the Ensembl browser. Underlined sequences represent the Sam68 binding site (SBS) within intron 6 and the SRSF1 binding site in exon 6. (B) The sequences of intron 6 and SBS and of the mutated version (SBSmut) of the synthetic RNAs generated. Affinity pulldown assays were performed with biotinylated RNA and streptavidin beads using 3T3-L1 cells and immunoblotted with anti-Sam68 antibodies. TCL, total cell lysate. A molecular mass marker is shown on the left.