FIG 9.

The expression of p31S6K1 contributes to the adipogenesis defects of Sam68-deficient mouse preadipocytes. (A) pRetrosuper or Sam68sh 3T3-L1 cells were transfected with siGFP (control), sip31-A, and sip31-B. The protein extracts were immunoblotted with the indicated antibodies. β-Tubulin is shown as the loading control. Molecular mass markers are shown on the left in kilodaltons. (B) The cells indicated in panel A were induced to differentiate for 4 days. Adipocyte differentiation was assessed by Oil Red O staining. (C) The mRNA levels of PPARγ, C/EBPα, and GLUT4 normalized to GAPDH were assessed by RT-qPCR. The data are expressed as relative values from differentiation days 0 and 4. Error bars represent ± standard deviations of the means (*, P < 0.05).