Fig. 1.

ANG II-induced hypertension is associated with increased renal cytochrome P-450 (CYP)1B1 activity and expression in female mice. Cyp1b1^+/+ and Cyp1b1^−/− mice were infused with vehicle or ANG II for 2 wk, as described in materials and methods. Animals were euthanized, and renal tissue was collected to measure CYP1B1 activity using the P450-Glo assay, as described in materials and methods. A: activity of CYP1B1 expressed as relative luminescence units (RLU). B: CYP1B1 protein expression was measured by Western blot analysis in renal tissue from vehicle- and ANG II-infused Cyp1b1^+/+ and Cyp1b1^−/− mice using ∼10 μg protein for loading, as described in materials and methods. CYP1B1 protein expression was normalized against β-actin, which was used as a loading control. au, Arbitrary units. Data are expressed as means ± SE; n = 3–5 for all experiments. *P < 0.05, ANG II vs. the corresponding value with vehicle.