Fig. 2.

Renal interstitial accumulation of α-smooth muscle actin (α-SMA) caused by ANG II infusion in Cyp1b1^−/− but not Cyp1b1^+/+ female mice. Cyp1b1^+/+ and Cyp1b1^−/− mice were infused with vehicle or ANG II for 2 wk, as described in materials and methods. A: ANG II infusion increased renal interstitial staining of α-SMA in Cyp1b1^−/− mice, which was minimized in Cyp1b1^+/+ mice. B: graph showing quantified data. Data are expressed as means ± SE; n = 3 for all experiments. *P < 0.05, ANG II vs. the corresponding value with vehicle; †P < 0.05, ANG II-infused Cyp1b1^−/− mice vs. ANG II-infused Cyp1b1^+/+ mice.