Fig. 7.
Infusion of ANG II decreased nitric oxide (NO) bioavailability and increased production of 3-nitrotyrosine (3-NT) in kidneys of Cyp1b1−/− but not Cyp1b1+/+ female mice. Cyp1b1+/+ and Cyp1b1−/− mice were infused with vehicle or ANG II for 2 wk. A: at the completion of the experiments, urine was collected, and excretion of nitrite/nitrate (NOx) was determined using a commercially available kit. B: increased staining of 3-NT, an indicator of peroxynitrite formation, was observed in kidney sections from ANG II-treated Cyp1b1−/− mice but not Cyp1b1+/+ mice. C: graph showing quantified data of 3-NT-positive staining. Data are expressed as means ± SE; n = 3 for all experiments. *P < 0.05, ANG II vs. the corresponding value with vehicle; †P < 0.05, ANG II-infused Cyp1b1−/− mice vs. ANG II-infused Cyp1b1+/+ mice.